Fig 2. Growth statistics of L1210 cells in a steady environment.

(A) Generation time distribution. The mean was 10.40 h, and the standard deviation was 3.07 h (n = 6, 033). (B) Survival function of generation time. Magenta points are the experimental data; the blue broken-line represents the curve fitted to the experimental data by a mixed shifted-exponential distribution (Eq 2); the green broken-line represents a curve of a single shifted-exponential distribution. The vertical line at τ = 13.31 h indicates the threshold of generation time above which the survival function becomes smaller than 0.06. (C) Correlation between the generation times of mother and daughter cells (n = 5, 537). (D) Autocorrelation of generation time. Error bars represent standard errors. (E) Division count distribution. The number of cell divisions in each lineage that stayed alive during the seven-day culture (n = 341) was counted. The red columns represent the distribution of division counts; the blue curve represents the estimated distribution of division counts, assuming that there is no trans-generational correlation between generation times. The latter was calculated by randomly sampling generation times from the experimental data. (F) Distribution of intra-lineage mother-daughter correlation of generation time. Spearman’s correlations were calculated for 447 single-cell lineages separately and shown as a histogram. The mean and standard deviation were 0.23 and 0.33, respectively.