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. 2021 Feb 1;16(2):e0245070. doi: 10.1371/journal.pone.0245070

Fig 2. Statistical analysis of the differentially expressed genes.

Fig 2

a. The phenotypes of cotton cotyledons with- or without EBR treatments after exposure to cold stress for 24 h. b. Total RNA extracted from ‘Lumianyan 28’ leaves. The numbers on the left represent the DNA marker ladder lengths (base pair, bp). L, DNA ladder. The numbers 1–6 represent RNA samples from the Cold+water_1, Cold+water_2, Cold+water_3, Cold+EBR_1, Cold+EBR_2, and Cold+EBR_3 treatments, respectively. c. A heatmap of Pearson correlation coefficients from comparisons of the Cold+water and Cold+EBR samples (three replicates of each). The color bar from blue to red indicates the correlation coefficients from 1 to 0. d. Volcano plot showing the DEGs in the two different libraries. The q<0.05 was used as the threshold to determine the significance of the DEGs. Red dots represent up-regulated genes, green dots represent down-regulated genes, and the black dots indicate transcripts that did not change significantly between the Cold+water vs. Cold+EBR treatment libraries.