Fig 2. K25 is essential for reverse transcription and infection.

(a&b&c) Infectivity of WT HIV and selected mutants. Infectivity is measured using an IncuCyte and determined as the proportion of cell area that is GFP +ve at a given viral dose (in ng RT). (d) Matching infectivity (GFP +ve cell area) and reverse transcription (strong-stop, RU5, at 4 hours post-infection) of chimeric viruses produced with an increasing ratio of WT:K25A Gag. (e) ERT assay measuring the synthesis of strong-stop DNA in the presence of DNase and at increasing concentrations of dNTPs. WT and K25A cores with and without IP6 (50 μM). Data is average of three biological replicas and has been normalized to the copies of RU5 measured in the absence of dNTPs. Error bars in infection experiments depict mean ± SD of three replicates from one experiment representative of three independent experiments.