Skip to main content
. 2021 Feb 1;17(2):e1009164. doi: 10.1371/journal.ppat.1009164

Fig 6. K25 is required for IP6-mediated assembly of HIV CA.

Fig 6

In vitro assembly reactions were monitored in real-time be measuring the absorbance at 350 nm. (a&b) Assembly reactions using 75 μM capsid protein and 2.5 M NaCl in 50 mM MES pH 6.0 (c) Negative stain EM images of material taken from (a&b). Scalebar: 200 nm. (d) Assembly reactions using 100 μM WT and mutant capsid protein with 1.25 mM IP6 and (e) 200 μM WT and mutant capsid protein with 6 mM IP6 in 50 mM MES pH 6.0, 40 mM NaCl (f) Negative stain EM images of material taken from (d&e). Scalebar: 200 nm (g) Assembly reactions of 250 μM WT or K25A CA in 50 mM MES pH 6, 100 mM NaCl and a range of IP6 concentrations up to 10 mM. (h) Negative stain EM images of material taken from (f).