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. 2021 Jan 20;17(1):e1009221. doi: 10.1371/journal.ppat.1009221

Fig 5. Comparison of the response of two oral epithelial cell lines to C. albicans infection and EGFR inhibition.

Fig 5

(A) Adherence (cell-association) and endocytosis of wild-type C. albicans strain SC5314 by OKF6/TERT-2 and TR146 oral epithelial cell lines. (Orgs/10 HPF, organisms per 10 high-power fields) (B) Comparison of the effects of gefitinib (GEF) versus diluent (ctrl) on C. albicans adherence to and endocytosis by OKF6-TERT-2 and TR146 cells. (C) Time course of C. albicans-induced damage to OKF6-TERT-2 and TR146 cells. (D) Extent of damage to OKF6/TERT-2 and TR146 cells caused by C. albicans and Triton X-100 (TX-100) after 24 h incubation. (E) Comparison of the extent of phosphorylation on the indicated tyrosine residues of EGFR in unstimulated OKF6/TERT-2 and TR146 cells. (F) Phosphorylation of EphA2 and EGFR in TR146 cells after 30 and 60 min post-infection (p.i.) with C. albicans. Results in (E and F) are representative immunoblots from 3 independent experiments. Densitometric quantification of all 3 immunoblots is shown in S11 Fig. (G) Comparison of the basal release of the indicated inflammatory mediators by uninfected OKF6-TERT-2 and TR146 cells. Results are the ratio of TR146 cells to OKF6/TERT2 cells. (H) Effects of gefitinib on C. albicans-induced production of the indicated pro-inflammatory mediators by TR146 cells. Graphs show the results of 3 experiments, each performed in triplicate (A-D) or duplicate (G and H). The data in (A, B, and D) were analyzed with the Mann-Whitney test, and the data in (C, G, and H) were analyzed by the Kruskal-Wallis test corrected for multiple comparisons. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****p, < 0.0001.