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. 2021 Jan 28;14:725–736. doi: 10.2147/OTT.S277951

Figure 5.

Figure 5

Association between lnc-UCID and Snail mRNA blocks the inhibitory effect of miR-122, miR-203, miR-30b, miR-34a and miR-153 on Snail. (A) Dual-luciferase assays were conducted to analyze the effect of lnc-UCID and Snail mRNA interaction on the miR-122, miR-203, miR-30b, miR-34a and miR-153 binding on Snail 3ʹ-UTR. **P<0.01, compared to miR-NC+sh-NC group; ^^P<0.01, compared to miR-inhibitors+sh-NC group. (B) The level of Snail was assessed by qRT-PCR and Western blot assays. **P<0.01, compared to miR-NC+sh-NC group; ^^P<0.01, compared to miR-inhibitors+sh-NC group. (C) The effect of lnc-UCID on Snail expression was assessed by qRT-PCR and Western blot assays. **P<0.01, compared to vector+sh-NC group; ^^P<0.01, compared to Snail+sh-NC group.