Fig. 3. Fluorescence imaging analysis of RFP expression. (A) Transfection process of RFP-rAAV plasmid in HEK293T cells. The RFP expression was detected by fluorescence imaging at 0, 24, 48, 72 h. The first row of pictures were taken in brightfield conditions. (B) Infection process of HEK293T cells by RFP-rAAV detected at 0, 24, 48, 72 h under a fluorescent microscope. The first row of pictures were taken in brightfield conditions.

RFP, red fluorescent protein; rAAV, recombinant adeno-associated virus.