Figure 7.

Hemin causes senescence in neurons; PEG-HCC reduces hemin-induced senescence. (a and b) Representative light microscopy images of staining for β-galactosidase, a marker of cellular senescence, in differentiated neurons treated with hemin (5 or 10 μM, 24 h). Quantitation of β-galactosidase-positive cells is shown in the histogram. (c) Real-time mRNA quantitation of senescence-associated genes indicated senescence-associated secretory phenotypes. (d and e) Effect of PEG-HCC on hemin-induced senescence. Neurons were treated with hemin (10 μM) with or without PEG-HCC for 24 h. PEG-HCC reduced hemin-induced senescence. Results are represented as mean ± SEM from samples assayed in triplicate (for each experiment) and 3 independent experiments. *p < 0.01, **p < 0.05. Scale bar, 10 μm.