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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: ACS Nano. 2020 Feb 21;14(3):2827–2846. doi: 10.1021/acsnano.9b05821

Figure 9.

Figure 9.

DEF-HCC-PEG alleviates genome damage and senescence in experimental ICH model. (a–d) LA-PCR of total DNA extracted from 6 untreated and 6 DEF-HCC-PEG-treated ICH mouse brains. Untreated mice were treated with saline. Left hemisphere served as non-ICH control in each mouse brain, while right hemisphere was induced with ICH as described in Methods. Two untreated (mouse #1 and #2) and two DEF-HCC-PEG-treated ICH mouse brains (mouse #3 and #4) are represented in the gel image. (a and b) Nuclear (7.4 kb) and (c and d) mitochondrial DNA (10 kb) were used as long amplicons (LA), while 0.2 kb within the LA region was used as a short amplicon. Picogreen-based quantitation of amplified DNA from 6 mice, expressed as percent (%) of DNA integrity in the histograms. (e) qRT-PCR analysis to measure expression of senescence-associated factors’ mRNA levels from sham control, ICH-induced, and ICH-induced along with DEF-HCC-PEG-treated mice (n = 6). *p < 0.01, **p < 0.05.