Fig. 2. Protein expression levels of Notch1 are reduced in 8-week-old male Notch1 antisense transgenic (NAS) mice compared to control mice. (A) Protein expression levels of Notch1, 2, 3, and 4 in the liver by Western blotting and (B) The relative ratio of protein expression level of Notch1, 2, 3, and 4 in two groups. Equal protein loading was confirmed by β-actin (control, n=1; NAS, n=2).