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. 2020 Dec 4;26(2):443–455. doi: 10.1038/s41380-020-00960-8

Fig. 2. Visualizing spatial transmitter diffusion with genetically encoded sensors.

Fig. 2

A Schematic of stimulation-imaging experiments in acute mouse amygdalar slices. LA Lateral amygdala. B Three-dimensional spatiotemporal ΔF/F profiling of an iGluSnFR expressing amygdalar neuron to local electrical stimuli. Note one isolated release site indicated by pink arrow in B. C Pixel-wise maximal ΔF/F plot at the isolated release site indicated by the pink arrow in B. Fitting the data points in this plot with a single exponential decay function (pink line) yields an estimated glutamate spread length constant of 0.65 µm. Note the collection of exemplary animal experimental data made with the recently published project [32]. D Schematic of stimulation-imaging experiments in acute mouse brainstem slices. NTS nucleus tractus solitarius. E Three-dimensional spatiotemporal ΔF/F profiling of an GRABNP expressing NST neuron to local electrical stimuli. Note one isolated release site indicated by pink arrow in E. F Pixel-wise maximal ΔF/F plot at the isolated release site indicated by the pink arrow in E. Fitting the data points in this plot with a single exponential decay function (pink line) yields an estimated neuropeptide spread length constant of 0.91 µm. Note the collection of exemplary animal experimental data made with the recently published project [32].