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. 2020 Dec 4;26(2):443–455. doi: 10.1038/s41380-020-00960-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Schematic of stimulation-imaging experiments in ex vivo mouse basolateral amygdala (BLA) preparation. B Fluorescence responses of an iAChSnFR expressing amygdalar neuron evoked by a prolonged train of 1920 stimulating pulses at 16 Hz. Plots of cumulative (C) and individual (D) releases against stimulus numbers enable estimations of the readily releasable pool size, refilling rate, release probability of cholinergic synapses at the amygdalar neuron. Note in C a straight cyan line (f_(x) = 0.081 ● x + 0.0068) fitted to the late points of blue cumulative trace with its y-axis intercept and slope taken as the pool estimate and vesicle refill rate, respectively, and in D cyan trace representing averages of blue individual releases, corresponding to the release probability. Note the collection of exemplary animal experimental data made with the recently published project [32].