FIGURE 1.

Gating strategy of HSPCs and their subpopulations in the PBMCs. (A) Circle all PBMCs and exclude debris (P1). (B) Dead cells were excluded using 7-AAD (P2). (C) Gating on CD34⁺ cells in SSC-CD34 plot. (D) Subdivide CD34⁺ cells into CD34⁺CD38⁺ and CD34⁺CD38⁻ compartments. (E) Within the CD34⁺CD38⁺ compartment, CD135 and CD45RA expression defined CMPs, GMPs, and MEPs. (F) Within the CD34⁺CD38⁻ compartment, CD90 and CD45RA expression defined HSCs and MPPs. HSPC, hematopoietic stem and progenitor cell; PBMC, peripheral blood mononuclear cell; 7AAD, 7-aminoactinomycin D; SSC, side scatter; CMP, common myeloid progenitor; MEP, megakaryocytic–erythroid precursor; GMP, granulocyte–monocyte progenitor; HSC, hematopoietic stem cell; MPP, multipotential progenitor.