Fig. 2.

sf-RON promotes cell proliferation via enhancing glycolysis in GC cells. a Overexpression effects of sf-RON and RON in MKN-45 and GTL-16 cells were detected by western blotting assay. b Overexpression of sf-RON promoted cell growth in MKN-45 and GTL-16 cell lines compared with control cells using CCK-8 kit. c–e sf-RON enhanced the glucose consumption, glucose uptake, lactate uptake, and ATP uptake dramatically in MKN-45 and GTL-16 cell lines when compared with controls. f sf-RON increased the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) dramatically in gastric cells when compared with controls. Error bars = 95% CIs. The experiments were repeated three times, and a representative experiment is shown. g sf-RON induced the expression levels of GLUT1, LDHA, and HK2 by western blotting assay and qRT-PCR in MKN-45 and GTL-16 cells. **P < 0.01. h Expression of indicated protein in MKN-45 and GTL-16 cells were detected by western blotting assay