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. 2021 Jan 19;10:607349. doi: 10.3389/fonc.2020.607349

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Copyright © 2021 Kulasinghe, Taheri, O’Byrne, Hughes, Kenny and Punyadeera

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparative staining across two multiplex technologies. (A) H&E stain of head and neck squamous cell carcinoma (HNSCC) tumor tissue; (B) highlighting the tumor region; (C) Opal four-color IHC staining (PerkinElmer) of the HNSCC tissue section for cytokeratin (green), CD8 (yellow), PD-L1 (red), DAPI (blue); (D) a tumor ROI imaged at 20x; (E) indicative brown stains of the individual channels for CK, CD8, and PD-L1; (F) whole slide imaged with visualization markers for ROI selection on the Nanostring GeoMx DSP 12 regions of interest (ROI) selection in multiple tumor regions; (G) barcode counts per ROI across the visualization markers CD8, CD3, and pan-cytokeratin.