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. 2021 Jan 19;10:562823. doi: 10.3389/fonc.2020.562823

Figure 5.

Figure 5

TDO2 promoted EMT process via Kyn-AhR pathway. (A) The relative expression of IDO in LM3 andHuh7 compared to normal liver cell LO2, and in sh-Tdo2 Huh7 cells compared to sh-Scramble Huh7 cells, verified by qRT-PCR. (B) The concentration of Trp and Kyn in the supernatant of sh-scramble and sh-TDO2 Huh7 cells and the ratio of Kyn/Trp in the supernatant of Huh7 cells treated with TDO2 inhibitor 680C91. The concentration of Trp and Kyn was measured by HPLC. (C) Relative expression levels of AhR and its target gene CYP1b1 in the indicated cells. (D, E) Translocation of AhR activated by exogenous Kyn observed by Western blot and laser confocal fluorescence microscopy, Scale bar = 25 μm. (F) Scramble cell assay and Transwell metastasis and invasion assay of sh-Tdo2 Huh7 cells treated with PBS, Kyn (50 μM) and Kyn (50 μM) combined with AhR inhibitor CH-223191 (10 μM), Scale bar = 200 μm. (G) Relative expression levels of E-cadherin, N-cadherin, Vimentin and MMP9 in the sh-Tdo2 Huh7 cells treated with Kyn and AhR inhibitor measured by Western Blot. *P < 0.05; **P < 0.01; ***P < 0.001.