Table 2.
The full factorial design matrix showing the observed amylase activity of P. thermoglucosidasius Pharon1 exerted by the design trails
| Run | Coded parameters | Mean amylase activity (U/mL/min) | ||||
|---|---|---|---|---|---|---|
| A | B | C | D | Observed | Expected | |
| 1 | 8 | 50 | 96 | Aerobic | 0.0 | 0.3 |
| 2 | 5 | 50 | 96 | Anaerobic | 18.2 | 18.5 |
| 3 | 8 | 50 | 24 | Anaerobic | 0.0 | 0.3 |
| 4 | 5 | 75 | 96 | Anaerobic | 6.1 | 5.8 |
| 5 | 8 | 50 | 24 | Aerobic | 0.0 | − 0.3 |
| 6 | 5 | 75 | 24 | Anaerobic | 2.1 | 2.4 |
| 7 | 8 | 75 | 96 | Anaerobic | 0.0 | 0.3 |
| 8 | 5 | 50 | 24 | Anaerobic | 5.0 | 4.7 |
| 9 | 8 | 75 | 96 | Aerobic | 0.0 | − 0.3 |
| 10 | 5 | 75 | 96 | Aerobic | 17.4 | 17.7 |
| 11 | 5 | 50 | 24 | Aerobic | 32.0 | 32.3 |
| 12 | 8 | 75 | 24 | Aerobic | 0.0 | 0.3 |
| 13 | 8 | 75 | 24 | Anaerobic | 0.0 | − 0.3 |
| 14 | 5 | 50 | 96 | Aerobic | 59.4 | 59.1 |
| 15 | 8 | 50 | 96 | Anaerobic | 0.0 | − 0.3 |
| 16 | 5 | 75 | 24 | Aerobic | 3.3 | 3.0 |
P. thermoglucosidasius Pharon1 was inoculated in M4 medium [7] containing 1% soluble starch. Runs were triplicated, and the mean amylase activity was calculated