Fig. 8.

Administration of CD163 CRISPR knockout prior to ICH abolished the effect of rCCL17 at 72 h after ICH. (A) WB bands of CCR4, p-ERK1/2, ERK1/2, Nrf2, CD163, and actin; (B–E) quantitative analysis of CCR4, p-ERK/ERK, Nrf2, and CD163 expression; (F) modified Garcia test; (G) forelimb placement test; (H) corner turn test. *p < 0.05 versus sham, @p < 0.05 versus ICH + vehicle, #p < 0.05 versus ICH + rCCL17, $p < 0.05 versus ICH + rCCL17 + CD163 CRISPR, &p < 0.05 versus ICH + CRISPR diluent. Mean ± SD, one-way ANOVA, Tukey test, n = 6/group. p-ERK1/2, phosphorylated extracellular regulated protein kinases 1/2; ERK1/2, extracellular regulated protein kinases1/2