Fig. 6.

The expression pattern of Th1/Th2/Th17/regulatory transcription factors and cytokines is not altered in the CNS of DMH1-treated mice. (a) The expression of the transcription factors TBX21 (Th1), GATA3 (Th2), RORγT (Th17), and FOXP3 (Treg) and the expression of MRC1 (M2 macrophages), IFN-γ, TGF-β, IL-10, IL-4, and IL-17A were analyzed by qPCR in spinal cords of vehicle- and DMH1-treated mice. Six spinal cords from the vehicle group and seven samples from the DMH-1 group were included, except for TBX21 (five samples in the vehicle group) and IL-10 (five samples in the vehicle group and six samples in the DMH1 group). (b) The expression of the transcription factors TBX21 (Th1), GATA3 (Th2), RORγT (Th17), and FOXP3 (Treg) and the expression of MRC1 (M2 macrophages) were analyzed in the inflammatory infiltrate obtained from freshly isolated spinal cords of vehicle- and DMH1-treated mice. Six samples per group were included, with the exception of GATA3 (five samples in the DMH1 group). Data represent the mean ± standard deviation (SD). Comparisons between means were performed using mixed models