Fig. 2.

FTH1 was downregulated in the 6-OHDA model of PD. Morphological changes in nerve cells and protein expression were analyzed in the substantia nigra (SN) tissue from the normal, sham, and model groups. (A) HE staining (magnification ×4 and ×20). (B) Immunohistochemical staining of α-syn, TH, and FTH1 (magnification ×4 and ×20). (C) Western blot analysis of the expression of TH, α-syn, and FTH1. The data were normalized to the control group values. (D) PC-12 cells were untreated or treated with 6.3, 12.5, 25.0, 50.0, or 100.0 μM 6-OHDA for 24 h, and cell viability was evaluated by the MTS assay. (E) PC-12 cells were untreated or treated with 40 μM 6-OHDA for 12, 24, or 48 h, and cell viability was evaluated by the MTS assay. (F) PC-12 cells were untreated or treated with 20, 40, or 60 μM 6-OHDA for 24 h. The protein expression of TH, α-syn, and FTH1 was determined by Western blotting. The data are expressed as means ± S.D.; n ≥ 3 per group for all the studies (t test, **p < 0.01, ***p < 0.001). The data were normalized to the control group values