Fig. 3.

Fluorescence detection of living and dead cells. PC-12 cells were treated with 6.3, 12.5, 25.0, 50.0, or 100.0 μM 6-OHDA for 24 h. PI was used to detect dead cells (red), and calcein-AM was used to detect living cells (green). The cells were imaged under a fluorescence microscope