Fig. 4.

FTH1 mediated cytoprotective activity and altered protein expression in the cell model of PD. PC-12 cells were treated with 6-OHDA (40 μM) for 24 h to establish a cell model of PD. (A) PC-12 cells were transfected with FTH1 siRNA or siRNA control (NC). (B) PC-12 cells were infected with FTH1 overexpression lentivirus or control vector lentivirus (VEC). The expression of FTH1 was analyzed using Western blotting. The effect of FTH1 knockdown (C) or overexpression (D) on cell viability was determined using the MTS assay. The expression of TH in FTH1 knockdown (E) or FTH1-overexpressing (F) PC-12 cells was analyzed using Western blotting (n > 3 assays, t test, *p < 0.05, **p < 0.01). The data were normalized to the control group values