Fig. 7.

FTH1 mediated ferroptosis in PC-12 cells. The expression of GPX4 and FTH1 was evaluated in PC-12 cells treated with the knockdown (A) or overexpression (B) vector combined with 40 μM 6-OHDA. FTH1 expression was analyzed using Western blotting. The data were normalized to the control group values. A FeRhoNox-1 fluorescent imaging probe was employed for ferrous ion imaging of PC-12 cells treated with the knockdown (C) or overexpression (D) vector combined with 40 μM 6-OHDA. All data are representative of at least 3 independent experiments