Fig. 5.

mPDM608 treatment spares dopaminergic neurons following MPTP intoxication. (a) Representative immunohistochemical sections of TH⁺/Nissl⁺ dopaminergic neurons within the substantia nigra of mice treated with either PBS, MPTP alone, or MPTP and mPDM608 at doses of 0.1, 0.3, 1.0, 3.0, 5.0, 10.0, 15.0, 20.0, 25.0, or 30.0 mg/kg; MPTP and a single injection of 0.1 mg/kg rGM-CSF (1×); or 5 injections (5×) of 0.1 mg/kg rGM-CSF (scale bar = 500 μm). (b) Stereological quantification of total number of surviving dopaminergic (TH+/Nissl+) and nondopaminergic (TH⁻/Nissl⁺) neurons within the substantia nigra following MPTP intoxication. Differences in means (± SEM, n = 7) were determined in which p < 0.05 compared with groups treated with ^aPBS or ^bMPTP. Mean percent remaining total neuron number is indicated on each treatment bar