Fig. 5.

URA Restricted Th17 polarization in vitro. (a) Effect of 1 and 5 μM URA on differentiation of TCR^MOG 2D2 T cell was analyzed using flow cytometry under Th17 polarization in vitro. (b) The percentage of Th17 cells in the CD4⁺ subset was analyzed by intracellular staining of IL-17. (c) Supernatants derived from Th17 cell cultures were analyzed for the level of IL-17 production. (d) Relative expression of Th17 family genes of Th17 cells under URA treatment (5 μM). The relative expression was calculated by log2 of − ΔΔCt values from triplicate of PCR. Symbols represent mean ± SEM. Data were determined by unpaired Student's t test (b, c), **p < 0.01, ***p < 0.001.