Fig. 7.

URA inhibited Th17 differentiation by targeting AhR in vitro. (a) Molecular docking diagram of URA and AhR. (b) Schematic diagram of the interaction between URA and amino acid residues. (c) Statistical table of URA and AhR interaction and energy score. (d) Expression of AhR target genes in IL-17 Family Signaling Pathway in the presence or absence of 5 μM URA. (e) Expression of AhR target genes in dendritic cells developmental lineage pathway in the presence or absence of 5 μM URA. (f) Splenocytes from C57BL/6 mice were polarized under Th17 conditions for 3 days in the presence of either Vehicle or AhR antagonist (CH223191, 10 μM) or URA (2 μM) or pre-treated with 10 μM CH223191 for 4 h and subsequently treated cells with 2 μM URA. The induction of ex vivo IL-17 was measured by flow cytometry analysis. One representative of Three independent experiments is shown.