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. 2021 Jan 30;7(1):e06134. doi: 10.1016/j.heliyon.2021.e06134

Figure 3.

Figure 3

HCV core protein activates the Rb-E2F pathway via PA28γ-mediated downregulation of p16 levels. (A, C) HepG2-vector and HepG2-core cells were transfected with the indicated amount of PA28γ expression plasmid, SC shRNA plasmid, PA28γ shRNA plasmid, and p16 expression plasmid along with an empty vector for 48 h, followed by western blotting. The uncropped versions of figures are presented in Fig. S3A. (B, D) HepG2-vector and HepG2-core cells were transfected with 0.2 μg of E2F1-luc along with the indicated amount of PA28γ expression plasmid, SC shRNA plasmid, PA28γ shRNA plasmid, and p16 expression plasmid, followed by luciferase assay. Results are shown as means ± standard deviation (SD) obtained from four independent experiments (n = 4).