Fig. 6.

APBC alters gene signatures in murine tumor tissue. (A) A heatmap showing the top enrichment clusters of up- and downregulated genes in the RNA-seq data of isolated tumor tissue taken from APBC (15 mg/kg, n = 3) treated tumor-bearing mice using Metascape analysis. Gray color indicates a lack of significance. (B) Network of functional and pathway enrichment analyses were performed for differentially expressed genes (DEGs) using the Metascape tool. The sizes of the nodes reflect the enrichment significance of the terms, terms with up-/downregulated genes are shown in blue/red, respectively. (C) GSEA enrichment plot of KEGG pathway for genes in each group. (D) Tumor infiltration proportion of immune cells in each sample using CIBERSORT estimation. CD8⁺ TILs from APBC therapy groups showed significant enrichment compared to vehicle-treated ones. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)