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. 2021 Feb 1;12:723. doi: 10.1038/s41467-021-20962-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a PAIII growth (F0) in response to treatment with MSC CM, heat-inactivated (HI) MSC CM, or proteinase-K (PK) treated MSC CM. b Cytokine Array of MSC CM. Black box indicates positive control (+ve), red box indicates IL-28. c RT-PCR analysis of PAIII (F0 and F2) of IL28Rα, IL-10Rβ and IL-28 expression. Molecular weights in base pairs are shown. d Growth of PAIII (F0) in MSC CM immune-depleted of IL-28 (MSC αIL-28). IgG was used as negative control (MSC IgG). Growth is expressed as a percentage of non-treated cells. e Treatment of PAIII F0 and F2 cell lines with the indicated concentrations of recombinant IL-28 (rIL-28) for 48 h. f Growth of IL-28Rα silenced (sh-IL28R) and scrambled control (sh-SCR) compared to parental PAIII cell lines. g, h Control (sh-SCR) and IL-28Rα (sh-IL28R) PAIII and DU145 growth in MSC CM or rIL-28 as measured by luminescence assay and relative light unit (RLU) measurement or MTT assay. Experiments were repeated on at least two (c, f, h) or three (a, e–i) occasions. Error bars represent the mean ± SEM. Statistical analyses were performed by one-way ANOVA with multiple comparisons at 95% CI (a, g, i) or unpaired t-test (e, f, h). Asterisks denotes statistical significance (**p ≤ 0.01, ****p ≤ 0.0001) while NS denotes not significant.