Figure 3.

Robust detection of plasma ccf-mtDNA variants by optimized captured-based mtDNA sequencing

(A–D) Comparison of (A) mtDNA mapping rate, (B) mtDNA sequencing depth, and (C) homoplasmic and (D) heteroplasmic mtDNA variant numbers between conventional capture-based sequencing procedure and the optimized capture-based sequencing procedure. Significance was determined by a paired t test. (E) Number of repeatable and unrepeatable mtDNA variants only detected by optimized capture-based sequencing. (F) Distribution of mtDNA variants only detected by optimized capture-based sequencing at different heteroplasmy level. (G) Substitution spectrum of mtDNA variants only detected by optimized capture-based sequencing.