Figure 4.

Reproducibility of captured-based mtDNA sequencing in detecting plasma ccf-mtDNA

(A) Consistency of homoplasmic and heteroplasmic mtDNA variants between two repeat experiments using 10 HCC plasma samples. (B) Comparison of heteroplasmic mtDNA variants in HCC plasma samples with lower consistency. (C) Correlation of mtDNA heteroplasmy level of all mtDNA variants between two repeat experiments. The significance of correlation was determined by Spearman’s rank correlation coefficient. (D) Percentage of heteroplasmic mtDNA variants with different substitution category of two repeat experiments. Rep, replicate.