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An alignment of the amino acid sequences of AML (GenBank ID: ADJ49206) with PETase from Ideonella sakaiensis (R103G/S131A mutant; PDB ID: 5XH3_A) and T.fusca cutinase TfCut2 (PDB ID:4CG1_A) using Clustal Omega server. The signal peptide of AML (GenBank ID: ADJ49206) was not included in the alignment. The important residues are highlighted as: catalytic residues, substrate binding subsite I, subsite II, extended loop, disulphide bond.
