Fig. 9.

A structural comparison of AML (pale orange) with PETase (pale green; PDB ID: 5XH3; R103G/S131A mutant with 1-(2-hydroxyethyl) 4-methyl terephthalate (HEMT) bound as PET analogue) and TfCut2 (purple; PDB: 4CG1). In the lower images the residues of subsite I (on left) and subsite II (on right) of the active site are shown as stick models. The superposition was performed in PyMol using C_α atoms with default parameters. The catalytic triad (Ser-His-Asp) of the enzymes were shown as a stick model while the overall structures were shown as cartoon model. The PET analog HEMT was presented as stick model in white. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)