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. 2020 Sep 20;20:132–146. doi: 10.1016/j.omto.2020.09.005

Figure 7.

Figure 7

EVs-miR-130b-3p Enhanced Lung Cancer Cell Tumorigenesis by Suppressing FOXO3 In Vivo

Nude mice were injected with H1299 cells pretreated with PBS, EVs, EVs-mimic-NC, EVs-miR-130b-3p mimic, EVs-miR-130b-3p mimic + OE-NC, or EVs-miR-130b-3p mimic + OE-FOXO3 for 12 h. (A) Tumor volume. (B) Tumor weight. (C) Tumor tissue morphology determined by H&E staining (400×) and Ki-67 expression determined by immunohistochemistry (200×). (D) FOXO3, Keap1, NFE2L2, and TXNRD1 expression in tumor tissues determined by western blot analysis. (E) miR-130b-3p expression determined by qRT-PCR. ∗p < 0.05 compared to mice injected with H1299 cell pretreated with PBS. #p < 0.05 compared to mice injected with H1299 cell pretreated with EVs-mimic-NC. &p < 0.05 compared to mice injected with H1299 cell pretreated with EVs-miR-130b-3p mimic + OE-NC. The data were shown as means ± standard deviations. Comparisons among multiple groups were analyzed by Tukey’s test-corrected one-way ANOVA. Variables were analyzed at different time points using Bonferroni-corrected repeated-measures ANOVA.