Figure 4.

Treatment with the HCA-EFZP-IL-12 vector results in immunological memory in treated animals

(A) Rechallenge experiment performed with the long-term survivors identified in Figure 1D. The long-term survivors in the HCA-EFZP-IL-12-treated group (N = 3) were subjected to rechallenge with K7M2 cells and compared with PBS-treated control mice (N = 5). Kaplan-Meier survival curves were constructed, and the p value was calculated with the log-rank test (p = 0.0123). (B) Analyses of tumor burden development in the PBS group (control group) and HCA-EFZP-IL-12-treated group. Tumor volume in mouse tibias was measured on different days until the end of the experiment. (C) Representative macroscopic H&E images of the tibias and lungs from a long-term survivor. Note that the tissues are completely normal.