Figure 5.

Local HCA-EFZP-IL-12 administration induces changes in the osteosarcoma microenvironment in the long term

(A−C) Sarcoma-bearing mice were treated as described in Figure 1 and sacrificed on day 80. Tumor samples were collected for analysis of CD31 (A), F4/80 (B), and GR1 (C). Upper panel: representative images (scale bar, 200 μm). Lower panels: quantification of the stained area as a percentage of the total area in bones and lungs (CD31, p = 0.01 and 0.02, respectively; F4/80, p = 0.001 and 0.05, respectively; and GR1, p = 0.001 and 0.2, respectively). Mann-Whitney test.