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. 2020 Dec 3;20:94–104. doi: 10.1016/j.omto.2020.11.006

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Peripheral blood mononuclear cell (PBMC) counts and markers

Blood was drawn pre- and post-adenovirus injection (day 1). PBMCs were harvested and frozen from each patient. Cells were thawed; stained with antibodies against CD3, CD56, CD4, CD8, CD69, CD45(RO), Ki67, and Tim3; and subjected to flow cytometry. All data are reported as group mean fold-increase over baseline blood collected prior to adenovirus injection, which occurred on day 1. Error bars show standard deviation. Day 21 data for cohort 1 were limited to patient 1 only. Samples from cohort 1 are shown in panels A-C; cohort 2 in panels D-F; and cohort 3 in panels G-I.