Figure 1.
IBP inhibits the growth of lung cancer cells
(A) A549, H460, H1299, and MLE-12 cells were incubated in the presence of IBP at different concentrations (0, 1, 2, 5, 10, 15, 20, 30, 40, and 50 μg/mL) for 48 h. Cell viability was assessed by MTT assay at a wavelength of 570 nm. (B) A549 and H460 cells were incubated in the presence of IBP in the indicated concentrations for 48 h. Cell viability was assessed by EdU incorporation assays. (C) A549 and H460 were cultured in the indicated concentrations of IBP for 14 days. Cell clone number was counted. (D) A549 and H460 cells were treated with IBP as (C), and apoptotic cells were assessed by TUNEL staining. (E–G) 3 × 107 of A549 cells per mouse was subcutaneously inoculated in the back of BALB/c mice. 1 or 10 mg/kg of IBP was administered by intraperitoneal injection every 2 days. Mice were sacrificed on day 12, and tumors were excised, photographed, and weighted. (H) Ki67 expression in tumor xenografts was examined by IHC. Representative images were provided as indicated; scale bar, 50 μm. Data are mean ± SD from three independent experiments. One-way ANOVA (Tukey’s post hoc); ∗p < 0.05; ∗∗p < 0.01.