Figure 3.

IBP promotes autophagy flux in lung cancer cells

(A) A549 and H460 cells were treated with 10 μg/mL IBP for 48 h. The interaction among Beclin1, Atg14L, and Vps34 in indicated cells was determined by coimmunoprecipitation assay. (B) Interaction between Beclin1 and Bcl-2 in A549 and H460 cells was tested as in (A). (C–F) A549 and H460 cells were transfected with RFP-GFP tandem fluorescent-tagged LC3 (RFP-GFP-LC3) for 24 h, and then cells were treated with 10 μg/mL IBP alone or in combination with 10 μM 3-MA for 48 h. Formation of endogenous LC3 puncta in cells was detected by CLSM. Scale bars, 10 μm. Data are mean ± SD from three independent experiments. One-way ANOVA (Tukey’s post hoc); ∗p < 0.05; ∗∗p < 0.01.