Figure 2.
Grm3 expression in glioma-initiating cells
(A) qRT-PCR in single cells (N = 482) derived from freshly dissociated human glioblastomas (N = 6, Table S1). x axis: gene expression of indicated genes. y axis: GRM3 gene expression. Slopes of blue lines indicate correlations. (B) GRM3 gene expression sorted for CD133 and analyzed by Affymetrix U133 Plus 2 arrays in a publically available dataset.17 (C) Flow cytometry of indicated GSCs utilizing fluorescence-labeled antibodies to Grm3 and CD44 or immunoglobulin G (IgG) isotype controls. Numbers in gates indicate percentages.