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. 2020 Mar 27;28(2):135–146. doi: 10.3727/096504019X15719983040135

Figure 6.

Figure 6

Upregulation of LCN2 increased cell proliferation and inhibited cell apoptosis in ovarian cancer. (A, B) qPCR and Western blot assays were performed to examine LCN2 expression levels after 48 h of cell transfection with OE-LCN2, OE-NC, sh-LCN2 or sh-NC in SKOV3 and OVCAR3 cells (*p < 0.05, sh-LCN2 group vs. sh-NC group; #p < 0.05, OE-LCN2 group vs. OE-NC group). (C, D) MTT assay was carried out to detect cell proliferation (*p < 0.05, sh-LCN2 group vs. sh-NC group; #p < 0.05, OE-LCN2 group vs. OE-NC group). (E) Flow cytometry assay was used to detect cell apoptosis (*p < 0.05, sh-LCN2 group vs. sh-NC group; #p < 0.05, OE-LCN2 group vs. OE-NC group). (F) Western blot assay was used to detect the protein expression level of LCN2 after SKOV3 and OVCAR3 cells were transfected with OE-KCNQ1OT1, OE- KCNQ1OT1 + mimics, or the control vectors (*p < 0.05, OE-KCNQ1OT1 group vs. control group; #p < 0.05, OE-KCNQ1OT1 + mimic group vs. OE-KCNQ1OT1 group).