Figure 5.

The effects of VASH2 on AKT signaling in A549 and H358 cells. (A) Western blot was performed to measure the expression of ERK and AKT and their phosphorylated form in A549 cells after VASH2 plasmid transfection. (B) Western blot was performed to measure the expression of ERK and AKT and their phosphorylated form in H358 cells after VASH2 shRNA-1 transfection. (C) Western blot was performed to measure the expression of P-gp in A549 cells after VASH2 plasmid transfection plus AKT inhibitor LY294002. (D) Western blot was performed to measure the expression of P-gp in A549 cells after VASH2 plasmid transfection plus ERK inhibitor U0126. (E) MTT assay was performed to measure the inhibition rate in A549 cells after VASH2 plasmid transfection plus either ERK inhibitor U0126 or AKT inhibitor LY294002. (F) The IC₅₀ was calculated from (E). *p < 0.05.