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. 2020 May 29;28(3):311–319. doi: 10.3727/096504020X15801233454611

Figure 3.

Figure 3

HOXA11-AS exerted the tumor-promoting role via sponging the tumor suppressor miR-155. (A) Subcellular fractionation followed by quantitative real-time polymerase chain reaction (qRT-PCR) revealed that HOXA11-AS was mainly localized in the cytoplasm of FaDu cells. (B) The relative luciferase activity of the HEK293 cells cotransfected with pmirGLO-HOXA11-AS-WT or pmirGLO-HOXA11-AS-MUT and mimic miR-155 or negative control. (C) Cell proliferation assay revealed that mimic miR-155 significantly suppressed cell proliferation, while inhibitor miR-155 significantly enhanced cell proliferation, and the suppressive effect of HOXA11-AS knockdown on cell proliferation was reversed by adding inhibitor miR-155 in FaDu cells. (D) Fluoroblok Transwell migration assay revealed that mimic miR-155 significantly suppressed cell migration, while inhibitor miR-155 significantly enhanced cell migration, and the suppressive effect of HOXA11-AS knockdown on cell migration was reversed by adding inhibitor miR-155 in FaDu cells (all p < 0.0001). Data are presented as the mean ± SEM. All the assays were performed in triplicate. ****p < 0.0001.