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. 2020 May 29;28(3):225–236. doi: 10.3727/096504019X15753718797664

Figure 3.

Figure 3

S100A16 was a direct target of miR-6884-5p. (A) Schematic representation of S100A16 3′-untranslated regions (3′-UTRs) showing putative microRNA (miRNA) target site. (B) The protein levels of S100A16 were determined by Western blot in MKN45 and SGC-7901 cells transfected with miR-NC, miR-485-5p, miR-873-5p, or miR-6884-5p mimic, respectively. (C) The analysis of the relative luciferase activities of S100A16-WT and S100A16-MUT in GC cells. (D) Relative miR-6884-5p level in GC tissues and their corresponding adjacent normal tissues. (E) Relative miR-6884-5p levels analyzed by qRT-PCR in MKN45, SGC-7901, and FTE187 cells. (F) Pearson’s correlation analysis of the relative miR-6884-5p levels of and the relative S100A16 mRNA levels in GC tissues. All data are presented as mean ± SEM, n = 4. ##p < 0.01 versus miR-NC or anti-miR-NC. **p < 0.01, ***p < 0.001 versus normal tissues or FTE187.