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. 2020 Sep 1;28(4):357–369. doi: 10.3727/096504020X15844389264424

Figure 2.

Figure 2

Effects of miR-4792 on the proliferation and apoptosis in AML cells. HL-60 and Kg1a cells were transfected with miR-4792 mimic or miR-NC for 48 h. (A) The level of miR-4792 was determined by qRT-PCR assay. (B) Cell proliferation was assessed by BrdU-enzyme-linked immunosorbent assay (ELISA) assay. (C) The mRNA expressions of proliferating cell nuclear antigen (PCNA), CDK4, cyclin D1, and p27 were determined by qRT-PCR assay. (D, E) Cell apoptosis was measured by flow cytometric analysis of cells labeled with annexin V/propidium iodide (PI) double staining and nucleosomal degradation, respectively. (F) The activities of caspase 3 were determined by caspase 3 activity detection assay. All data are presented as mean ± SEM, n = 6. #p < 0.05, ##p < 0.01, ###p < 0.001 versus miR-NC.