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. 2020 Sep 1;28(4):357–369. doi: 10.3727/096504020X15844389264424

Figure 6.

Figure 6

Overexpression of kindlin-3 partially promoted cell proliferation, invasion, and EMT in miR-4792-overexpressing AML cells. HL-60 cells were cotransfected with miR-NC or miR-4792 mimic with pcDNA3.1 or pcDNA-kindlin-3 vector. (A) The protein expression of kindlin-3 was determined by Western blot assay. (B) Cell proliferation was assessed by BrdU-ELISA assay. (C) The mRNA expressions of PCNA, CDK4, cyclin D1, and p27 were determined by qRT-PCR. (D) The invasion was assessed by Transwell assay. (E) The expressions of MMP-2, MMP-9, and TIMP-1 were detected by Western blot assay. (F) Cell apoptosis was measured by nucleosomal degradation by using Roche’s cell death ELISA detection kit. (G) The activities of caspase 3 were determined by caspase 3 activity detection assay. All data are presented as mean ± SEM, n = 6. *p < 0.05, **p < 0.01 versus miR-NC; #p < 0.05, ##p < 0.01 versus miR-4792 mimic + pcDNA3.1.