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. 2020 Feb 7;28(1):51–63. doi: 10.3727/096504019X15619783964700

Figure 5.

Figure 5

The reciprocal repression of FEZF1-AS1 and miR-30a-5p and miR-30a-5p repression promoted CRC cell proliferation and migration. (A) The expression of miR-610, miR-4443, miR-107, and miR-30a-5p was detected by qRT-PCR after FEZF1-AS1 knockdown in HCT116 cells. (B) qRT-PCR analysis of FEZF1-AS1 expression in HCT116 cells transfected with miR-NC, miR-30a-5p mimic, miR-NC inhibitor, or miR-30a-5p inhibitor. (C) Bioinformatics was used to predict miR-30a-5p binding sites in FEZF1-AS1. (D) The indicated constructs were transfected into human embryonic kidney (HEK293T) cells and cotransfected with miR-30a-5p mimics, and then the relative luciferase activity was detected. (E) The effect of the overexpression of miR-30a-5p on the proliferation of HCT116 and SW480 cells was detected by MTS assay. (F) The effect of the overexpression of miR-30a-5p on the migration of HCT116 and SW480 cells was detected by Transwell assay. *p < 0.05.