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. 2020 Sep 1;28(4):385–398. doi: 10.3727/096504020X15861709922491

Figure 3.

Figure 3

CYLD differentially regulates melanoma cell survival and proliferation in a cell line-dependent manner. (A, B) Mel-CV and ME1007 cell lines transfected with indicated siRNAs and with or without z-VAD-fmk were subject to CellTiter-Glo assays (A) and clonogenic assay (B). Data shown are representative of three individual experiments or means ± SEM. n = 3. *p < 0.05; **p < 0.01; ***p < 0.001, Student’s t-test. (C) Whole-cell lysates from Mel-CV and ME1007 transfected with indicated siRNAs were subjected to Western blotting. Data shown are representative of three individual experiments. (D) ME4405 cells transfected with indicated siRNA were subjected to 5-bromo-2′-deoxyuridine (BrdU) incorporation assays. Data shown are representative of three individual experiments or means ± SEM. n = 3. *p < 0.05. (E, F) ME4405 and Mel-FH cells transfected with indicated siRNAs were subjected to cell cycle distribution (E) and Western blotting (F). Data shown are representative of three individual experiments or means ± SEM. n = 3. *p < 0.05; **p < 0.01; ***p < 0.001; ns, p > 0.05, Student’s t-test. (G) Mel-FH cells were transfected with indicated siRNA and subjected to Western blotting. Data shown are representative of three individual experiments. (H–J) IgR3, Mel-JD, and MM200 cell lines transfected with indicated plasmids were subjected to Western blotting (H), CellTiter-Glo assays (I), and clonogenic assay (J). Scale bar: 1 cm. Data shown are representative of three individual experiments or means ± SEM. n = 3. *p < 0.05; **p < 0.01; ***p < 0.001; ns, p > 0.05, Student’s t-test. (K, L) HEMn-MP melanocytes transduced with indicated shRNAs (K) or cDNA (L) were subjected to CellTiter-Glo assays. Data shown are means ± SEM. n = 3. **p < 0.01; ns, p > 0.05, Student’s t-test.