FIG 6.

Suppression of cFOS upregulation and IBV replication by JNK and p38 inhibitors. (a) H1299 cells were infected with IBV at an MOI of ∼2 and treated with SP600125 at the indicated concentrations or with the same volume of DMSO at 2 hpi. Protein samples were harvested at the indicated time points and subjected to Western blot analysis using the indicated antisera. Beta-actin was included as the loading control. Sizes of protein ladders in kilodaltons are indicated on the left. Significance levels are presented by the P value (*, P < 0.05; ***, P < 0.001). (b) H1299 cells were treated with SB-203580 as described above for panel a. Protein samples were harvested at the indicated time points and subjected to Western blot analysis using the indicated antisera. Beta-actin was included as the loading control. Sizes of protein ladders in kilodaltons are indicated on the left. Significance levels are presented by the P value (**, P < 0.01; ****, P < 0.0001).