FIG 5.

The E4 family of mRNAs. (A) Previously characterized E4 mRNAs, with the likely proteins translated shown as gray boxes. The ORF3/4 mRNA is shown in light gray, since we could not detect reads corresponding to this mRNA in our Nanopore data. (B) Novel E4 mRNAs grouped based on the promoter position. The numbers of reads identified at 12, 24, and 36 hpi are shown in boxes to the right. Thick lines denotes exons, thin lines introns. Gray boxes indicate the proposed proteins expressed from respective mRNA. Poly-A, polyadenylation site. For a complete summary of the triple-verified E4 mRNAs, see Table S1.